Title
Author
DOI
Article Type
Special Issue
Volume
Issue
Article Menu
Export Article
More by Authors Links
Article Data
- Views 1274
- Dowloads 213
Original Research
Open AccessBlack stains in the mixed dentition: A PCR microbiological study of the etiopathogenic bacteria
Black stains in the mixed dentition: A PCR microbiological study of the etiopathogenic bacteria
- C. Saba1
- M. Solidani1
- F. Berlutti2
- A.Vestri3
- L. Ottolenghi1
- A. Polimeni4
1Department of Preventive Dentistry and Community Oral Health, School of Dentistry, University of Rome "La Sapienza", Italy
2Department of Microbiology, University of Rome "La Sapienza,", Italy
3Department of Experimental Medicine, School of Medicine and Surgery, University of Rome "La Sapienza,", Italy
4Department of Paediatric Dentistry, School of Dentistry, University of Rome "La Sapienza,", Italy
Abstract
The aim of this work is to emphasize that particular stains on the third cervical of the buccal and lin-gual surfaces in mixed dentition, called “black stain.” Previous research showed the microbiological etiology of this discoloration by chromogen bacterias. Our study shows bacteria spp involved in stains by means of PCR process and electrophoresis gel on the agarose medium. Sample was formed by 100 subject with black stain and 100 control subjects stain-free. A statistical analysis (SPSS 10.0) using X2 was performed in this study. Porphyromonas gingivalis and Prevotella melaninogenica, were not involved in both in black stain subjects and in the control. On the contrary, Actinomyces could be involved in the pigmentation process.
Cite and Share
C. Saba,M. Solidani,F. Berlutti,A.Vestri,L. Ottolenghi,A. Polimeni. Black stains in the mixed dentition: A PCR microbiological study of the etiopathogenic bacteria. Journal of Clinical Pediatric Dentistry. 2006. 30(3);219-224.
References
1. Bibby BG. A study of a pigmented dental plaque. J Dent Res 11: 855–72, 1931.
2. Commerel Von C. Zum problem der Karieresistenz. Dtsch Zahnaerztl 10: 1418–20, 1955.
3. Duerden B I. Pigment production by Bacteroides species with reference to sub-classification. J Med Microbiol 8: 113–25, 1974.
4. Georg L K, Robertstad G W, Brinkman S A. Identification of specie of Actinomyces. J Bacteriology (88) 2: 477–90, 1964.
5. Kamma JJ, et al. Profile of subgingival microbiota in children wihth mixed dentition. Oral Microbiol Immunol 15:103–111, 2000.
6. Koch M J, Bove M, Schroff J, Perlea P, Garcia-Goday F, Staehle HJ. Black stain and dental caries in schoolchildren in Potenza, Italy. J Dent Child Sept-Dec: 353–55, 2001.
7. James PMC. A survey of dental and gingival conditions in school children. Proceeding Royal Society Medicine 56: 620–24, 1963.
8. James PMC. Dental caries prevalence in relation to calculus, debris and extrinsic dental staining. Adv Fluorine Res Dent Caries Prev 3: 153–8, 1964.
9. Mellanby M, Coumoulos H. Teeth of 5-years old London school-children. Brit Med J 19: 565–70, 1946.
10. Mellanby M, Coumoulos H, Kelley M. Teeth of 5-years old London school-children (1955). Brit Med J 10: 318–22, 1957.
11. Onisi M, Nuckolls J. Actinomycetes and other pleomorphic organism recovered from pigmented carious lesions of human teeth. Oral Surg Oral Med Oral Path 7: 629–40, 1954.
12. Onisi M, Nuckools J. Description of Actinomycetes and other pleomorphic organism recovered from pigmented carious lesions of dentine of human teeth. Oral Surg 8: 910–30, 1958.
13. Oliver WW,Wherry WB. Notes on some bacterial parasites of the human mucous membranes. J Infect Dis 28: 341–4, 1921.
14. Pedersen PO. Farved Belaegninger i maelketandsaettet og deres Forhold til cariesforekomsten.” Tandlaegebladet (Danish Dental Journal) 50: 210–21, 1946.
15. Pickerill HP. A sign of immunity. Brit Dent J 44: 967–8, 1923.
16. Pickerill HP. The inoculation of the mouth with other organism. in:Prevention of dental caries and oral sepsis. 3th ed. London, Chap. 16 p. 263, 1923.
17. Reid JS, Beeley JA. Biochemical studies on the composition of gengival debres from children with black extrinsic tooth stain. Caries Res 10: 363–9, 1976.
18. Reid JS, Beeley JA, Mac Farlane TW.A study of the pigment pro-duced by Bacteroides melaninogenicus. J Dent Res 55: 1130, 1976.
19. Reid JS, Beeley JA, Mac Donald DG. Investigations into black extrinsic tooth stain. J Dent Res 56: 895–99, 1977.
20. Sato T, et al. Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism. Arch Oral Biol 247–252, 1998.
21. Schwabacher H, Lucas DR, Rimington C. Bacterium melanino-genicum – a misnomer. J Gen Miocrobiol 1: 109–20, 1947.
22. Slots J. The microflora of black stain on human primary teeth. Scand J Dent Res 82: 484–90, 1974.
23. Solidani M, Panetta F, Crisostomo L, Dall’Oca S, Polimeni A, Ottolenghi L. Pigmentazioni dentarie estrinseche in dentizione mista. Dental Cadmos 6: 13–21, 2004.
24. Solidani M, Panetta F, Crisostomo L, Dall’Oca S, Polimeni A, Ottolenghi L. Orletto pigmentato nero: protocollo diagnostico-terapeutico in dentizione mista. Dental Cadmos 3: 69–83, 2005.
25. Surdacka A. Chemical composition of the saliva in children and adolescent with black tartar. Czas Stomatol 10-12: 525–33, 1989.
26. Sutcliffe P. Extrinsic tooth stains in children. Dent Practit 17: 175–9, 1967
27. Theilade J, Pang KM. Scanning electron microscopy of black stain on human permanent teeth. Scanning Microscopy 1: 1983–9, 1987.
28. White PA, et al. Molecular characterization of an outer mem-brane protein of Actinobacillus actinomycetemcomitans belong-ing to the OmpA family. J Infect Immun 66: 369–372, 1998.
Abstracted / indexed in
Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.
Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.
Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.
JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.
Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.
BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.
Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.
Scopus: CiteScore 1.8 (2023) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.
Submission Turnaround Time
Conferences
Top