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Original Research

Open Access

Dental Pulp Stem Cells from Primary Teeth Quality Analysis: Laboratory Procedures

  • Saha R1,*,
  • Tandon S2
  • Rajendran R3
  • Nayak R4

1Pedodontics & Preventive Dentistry, ITS-CDSR, Muradnagar

2Pedodontics & Preventive Dentistry, UP Dental College & Research Center

3Manipal, Stempeutics, Manipal

4Pedodontics and Preventive Dentistry, MCODS, Manipal

DOI: 10.17796/jcpd.36.2.6j0w230354732454 Vol.36,Issue 2,March 2012 pp.167-174

Published: 01 March 2012

*Corresponding Author(s): Saha R E-mail: rooposhi@yahoo.com

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Abstract

Objectives: To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth. Materials and methods: Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow. Results: There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages. Conclusion: Isolation of cells from oral tissues is technique sensitive.

Keywords

Stem cell, inflamed pulp, deciduous teeth, stem cell marker, in vitro differentiation

Cite and Share

Saha R,Tandon S,Rajendran R,Nayak R. Dental Pulp Stem Cells from Primary Teeth Quality Analysis: Laboratory Procedures. Journal of Clinical Pediatric Dentistry. 2012. 36(2);167-174.

URL:

https://www.jocpd.com/articles/10.17796/jcpd.36.2.6j0w230354732454

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