In Vivo Evaluation of Lesion Sterilization and Tissue Repair in Primary Teeth Pulp Therapy Using Two Antibiotic Drug Combinations

Aim : The purpose of this study is to evaluate and compare the clinical and radiographic effectiveness of Ciprofloxacin, Minocycline, Metronidazole combination with Ciprofloxacin, Minocycline and Tinidazole combination when used for Lesion Sterilization and Tissue Repair in primary teeth. Method: 25 healthy children, visiting Dept. of Pediatric & Preventive Dentistry, D.A.P.M.R.V.Dental College, Bangalore, India, aged between 6 - 9 years who were having 30 infected primary teeth are selected and divided into 2 groups. In Group A, a mixture of 3mix-MP Ciprofloxacin, Metronidazole and Minocycline was placed on the floor of the pulp chamber covering the root canal orifices. In Group B a mixture of Ciprofloxacin, Tinidazole and Minocycline was placed as a layer on the floor of the pulp chamber. The procedure was completed in a single visit. Post operative clinical evaluation was done after 1,6,12 and 24 months. Postoperative radiographic evaluation was done at 6,12 and 24 months. Results: No statistically significant difference is observed between both the groups and a combination of Ciprofloxacin, Minocycline and Tinidazole antibacterial drugs can be used on teeth pulpally involved with physiologic root resorption. Conclusion: After a 24 Month follow up, we can conclude that primary teeth with the periradicular lesions, can be conserved by using combination of Ciprofloxacin, Minocycline and Tinidazole antibacterial drugs.

Lesion Sterilization and Tissue repair, Primary tooth, pulp therapy, Antibiotics, Ciprofloxacin, Minocycline, Tinidazole

1. Camp JH Pediatric Endodontic Treatment. In Cohen S. Burns RC, eds. Pathways of the pulp. 6th edn. St Louis MO. USA Mosby. 633-671: 1994.

2. Finn SB. Clinical Pedodontics, 4th ed. Philadelphia: W.B. Saunders Company; 201-219:1995.

3. Guideline on pulp therapy of primary and immature permanent teeth. American Academy of Pediatric Dentistry. Revised, Ref.No.3, 33(6), 194- 201: 2009.

4. Prabhakar.AR, Sridevi.E, Raju OS, Satish.V. Endodontic Treatment of Primary teeth using combinatio of antibacterial drugs. J Indian Soc Pedod Prevent Dent: Suppl: 26: S5-S10, 2008

5. Cruz EV, Kota K, Huque J, Iwaku M, Hoshino E. Penetration of propylene glycol into dentine. Int Endod Journal: 35: 330-336: 2002.

6. Alam T, Nakazawa F, Nakajo K, Umetsu H, Hoshino E. Susceptibility of Enterococcus Faecalis to a combinaion of Antibacterial drugs (3mix) invitro. J Oral Biosci : 47(4): 315-320: 2005.

7. David HW. Treatment of metronidazole resistant trichomonas vaginalis with tinidazole: case reports of three patients. Sexually Transmitted Diseases. 31 (6): 343-345: 2004.

8. Abott PV, Hume Wr, Pearman JW: Antibiotics and Endodontics. 35 (10): 50- 60: 1990.

9. Shafer WG, Hine MK, Levy BM, Tomich CE: A textbook of ral Pathology, W. B Saunders Company: 4th Edition.: 669, 1997.

10. O’Riordan MW, Coll J. Pulpectomy procedure for deciduous teeh with severe Pulpal necrosis. J Am Dent Assoc: 99: 480-482: 1979.

11. Takushige T, Cruz Ev, Moral A, Hoshino E. Endodontic Treatment of primary teeth using combination of antibacterial drugs. Int Endod J: 37: 132- 138: 2004.

12. Thomas PA, Bhat KS, Kotian KM. Antibacterial Properties of Dilute Formoacresol and Eugenol and Propylene Glycol. Oral Surg: 49: 166-170: 1980.

13. Rocha MJ, Cardoso M, Endodontic treatment of traumatized primary teeth – Part2. Dent. Traumatol: 20(6) : 314-326: 2004.

14. Hoshino E, Asgor MA, Yagi M, Garcia MCG, Cruz EV, Oyanagi H, Kota K. Oral Health program using LSTR 3 Mix- MP NIET Therapy: Pulp Cell Behaviour: Pulp Therapy and Endod: 1786: 2006.