Molecular Detection of Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis in Subgingival Plaque of Healthy Young Children

The aim of the present study was to evaluate the presence of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis) in subgingival plaque of healthy young children aged between 3 and 15 years using Polymerase Chain Reaction (PCR) and to compare their presence in children in their primary, mixed and permanent dentitions. Method: Subgingival plaque samples were collected from 120 healthy children and were grouped as Group I-Deciduous dentition, Group II- Mixed dentition and Group III- Permanent dentition, and were subjected to PCR assay. Results: Binomial test and Proportions test were used for statistical analysis. A. actinomycetemcomitans and P. gingivalis were detected in 5% and 35%, 12.5% and 20%, and 0% and 27.5% samples in group I, II and III respectively Conclusion: Both the microorganisms were present in group I and II. In group III only P. gingivalis was present. The results from this study do not necessarily pertain to differences in dentition but possibly to inter-individual differences

Periodontal disease, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Polymerase Chain Reaction

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