Physical and mechanical characterizations of experimental pit and fissure sealants based on bioactive glasses

Fissure sealants commonly exhibit weak mechanical and physical properties, potentially compromising their effectiveness in preventing dental caries. Therefore, this laboratory study aimed to synthesize and characterize experimental pit and fissure sealants in comparison with a commercially available sealant. Three different formulations of experimental pit and fissure sealing materials were synthesized using a blend of bisphenol A-glycidyl methacrylate (Bis-GMA) and triethylene glycol dimethacrylate (TEGDMA) monomers. The resin composition encompassed 70 wt.%, with 30 wt.% fillers comprising 10 wt.% silica in each composition, while 20 wt.%bioactive glasses. Specifically, group G1 employed Biomin F powder, group G2 utilized Biomin C powder, and group G3 incorporated S53P4 powder. The control group (GC) was a commercially available pit and fissure sealant (Seal-Rite). Ten disk-shaped specimens from each study group were fabricated (n = 10/group). The surface roughness, water contact angle, nanohardness (nH), elastic modulus (EM), water solubility and sorption were statistically evaluated using a one-way analysis of variance (p < 0.05). The surface roughness of the G1 & G2 groups and the water contact angle of G1, G2 & G3 groups were significantly lower compared to the control group (p < 0.05). However, nH, EM, water solubility, and sorption were notably higher in the control group compared to the experimental groups, except G1, which exhibited no significant difference from the control group (p > 0.05). The inclusion of micron-sized Biomin F powder in the experimental Bis-GMA/TEGDMA resin formulation demonstrated advantageous effects in reducing surface roughness and forming a lower contact angle without compromising the mechanical attributes.

Bioactive glass; Caries; Pit and fissure sealant; Physical properties; Mechanical properties

[1] Meeral PR, Prabakar J, Indiran MA, Ganesh J. Silver nanoparticles incorporated hydrophilic pit and fissure sealant-its preparation, characterization and assessment of shear bond strength. Journal of Population Therapeutics and Clinical Pharmacology. 2023; 30: 102–109.

[2] Splieth CH, Ekstrand KR, Alkilzy M, Clarkson J, Meyer-Lueckel H, Martignon S, et al. Sealants in dentistry: outcomes of the ORCA Saturday afternoon symposium 2007. Caries Research. 2010; 44: 3–13.

[3] Asefi S, Eskandarion S, Hamidiaval S. Fissure sealant materials: wear resistance of flowable composite resins. Journal of Dental Research, Dental Clinics, Dental Prospects. 2016; 10: 194–199.

[4] Ramamurthy P, Rath A, Sidhu P, Fernandes B, Nettem S, Fee PA, et al. Sealants for preventing dental caries in primary teeth. Cochrane Database of Systematic Reviews. 2022; 2: CD012981.

[5] Alkheraif AA, AlMufareh NA, AlQhtani FA, Asiri W, Abuhadi RI, Hamoud M, et al. Minocycline incorporated cobalt oxide nanoparticles in dental sealants: an in vitro study. Microscopy Research and Technique. 2022; 85: 2558–2566.

[6] Rashed T, Alkhalefa N, Adam A, AlKheraif A. Pit and fissure sealant versus fluoride varnish for the prevention of dental caries in school children: a systematic review and meta-analysis. International Journal of Clinical Practice. 2022; 2022: 8635254.

[7] Babu G, Mallikarjun S, Wilson B, Premkumar C. Pit and fissure sealants in pediatric dentistry. SRM Journal of Research in Dental Sciences. 2014; 5: 253–257.

[8] Osorio E, Osorio R, Davidenko N, Sastre R, Aguilar JA, Toledano M. Polymerization kinetics and mechanical characterization of new formulations of light‐cured dental sealants. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2007; 80B: 18–24.

[9] Nikolaidis A, Vouzara T, Koulaouzidou E. Pit and fissure nanocomposite sealants reinforced with organically modified montmorillonite: a study of their mechanical properties, surface roughness and color stability. Dental Materials Journal. 2020; 39: 773–783.

[10] Alrahlah A, Khan R, Al-Odayni A-B, Saeed WS, Bautista LS, Haider S, et al. Fabrication of Novel Pre-Polymerized BisGMA/silica nanocomposites: physio-mechanical considerations. Journal of Functional Biomaterials. 2023; 14: 323.

[11] Al-Odayni A, Alfotawi R, Khan R, Sharaf Saeed W, Al-Kahtani A, Aouak T, et al. Synthesis of chemically modified BisGMA analog with low viscosity and potential physical and biological properties for dental resin composite. Dental Materials. 2019; 35: 1532–1544.

[12] Lin C, Lin Y, Lai Y, Lee S. Mechanical properties, accuracy, and cytotoxicity of UV-polymerized 3D printing resins composed of Bis-EMA, UDMA, and TEGDMA. The Journal of Prosthetic Dentistry. 2020; 123: 349–354.

[13] Raszewski Z, Chojnacka K, Mikulewicz M, Alhotan A. Bioactive glass-enhanced resins: a new denture base material. Materials. 2023; 16: 4363.

[14] Habib E, Wang R, Zhu X. Monodisperse silica-filled composite restoratives mechanical and light transmission properties. Dental Materials. 2017; 33: 280–287.

[15] Khan AA, Siddiqui AZ, Syed J, Elsharawy M, Alghamdi AM, Matinlinna JP. Effect of short E-glass fiber reinforcement on surface and mechanical properties of glass-ionomer cements. Journal of Molecular and Engineering Materials. 2017; 5: 1740007.

[16] Marghalani HY. Effect of filler particles on surface roughness of experimental composite series. Journal of Applied Oral Science. 2010; 18: 59–67.

[17] Oliveira GUd, Mondelli RFL, Charantola Rodrigues M, Franco EB, Ishikiriama SK, Wang L. Impact of filler size and distribution on roughness and wear of composite resin after simulated toothbrushing. Journal of Applied Oral Science. 2012; 20: 510–516.

[18] Rupp F, Liang L, Geis-Gerstorfer J, Scheideler L, Hüttig F. Surface characteristics of dental implants: a review. Dental Materials. 2018; 34: 40–57.

[19] Khan AA, De Vera MAT, Mohamed BA, Javed R, Al‐Kheraif AA. Enhancing the physical properties of acrylic resilient denture liner using graphene oxide nanosheets. Journal of Vinyl and Additive Technology. 2022; 28: 487–493.

[20] Kalachandra S, Taylor D, DePorter C, Grubbs H, McGrath J. Polymeric materials for composite matrices in biological environments. Polymer. 1993; 34: 778–782.

[21] Kaur G. Clinical applications of biomaterials: state-of-the-art progress, trends, and novel approaches. 1st edn. Springer: Cham. 2017.

[22] Khan AA, Al-Kheraif AA, Al-Shehri AM, Säilynoja E, Vallittu PK. Polymer matrix of fiber-reinforced composites: changes in the semi-interpenetrating polymer network during the shelf life. Journal of the Mechanical Behavior of Biomedical Materials. 2018; 78: 414–419.

[23] Janaphan K, Hill R, Gillam D. In vitro evaluation of the abrasiveness of novel bioactive glass powders (Biominf®) on ivory dentine in air polishing procedures compared to selected reference powders. Journal of Dental and Maxillofacial Research. 2021; 4: 1–6.

[24] Khan AA, AlKhureif AA, Mohamed BA, Bautista LS. Enhanced mechanical properties are possible with urethane dimethacrylate-based experimental restorative dental composite. Materials Research Express. 2020; 7: 105307.

[25] Khan AA, Mohamed BA, Al-Shamrani SS, Ramakrishnaiah R, Perea-Lowery L, Säilynoja E, et al. Influence of monomer systems on the bond strength between resin composites and polymerized fiber-reinforced composite upon aging. The Journal of Adhesive Dentistry. 2019; 21: 509–516.

[26] Gonçalves F, Kawano Y, Pfeifer C, Stansbury JW, Braga RR. Influence of BisGMA, TEGDMA, and BisEMA contents on viscosity, conversion, and flexural strength of experimental resins and composites. European Journal of Oral Sciences. 2009; 117: 442–446.

[27] Rivera‐Torres F, Vera‐Graziano R. Effects of water on the long‐term properties of Bis-GMA and silylated-(Bis-GMA) polymers. Journal of Applied Polymer Science. 2008; 107: 1169–1178.

[28] Sideridou I, Tserki V, Papanastasiou G. Study of water sorption, solubility and modulus of elasticity of light-cured dimethacrylate-based dental resins. Biomaterials. 2003; 24: 655–665.

[29] Gajewski VE, Pfeifer CS, Fróes-Salgado NR, Boaro LC, Braga RR. Monomers used in resin composites: degree of conversion, mechanical properties and water sorption/solubility. Brazilian Dental Journal. 2012; 23: 508–514.