The effect of Matrigel in deciduous tooth pulp transplantation: an in vitro study

Background: Pulp regeneration in immature permanent teeth depends on whether deciduous teeth’s pulp can establish early blood supply connections with surrounding tissues after transplantation. This study aimed to explore the effect of Matrigel on early blood supply establishment after transplantation. Methods: Empty root canals of permanent teeth were prepared and divided into 3 groups (n = 18 each). Group A (Deciduous Pulp group): The pulp of deciduous teeth was extracted, transplanted into an empty root canal, and then implanted subcutaneously into nude mice. Deciduous pulp/Matrigel group as Group B, empty root group as Group C. Results: Histochemical and immunohistochemical examinations were performed 8 weeks after implantation. Both Groups A and B had pulp tissue and fibrous connective tissue interwoven within the root canals. Immunohistochemical staining revealed that human platelet endothelial cell adhesion molecule-1 (CD31) positive cells were scattered over pulp tissue area, while mice CD31 positive cells were scattered over connective tissue area. Meanwhile, human Nestin immunohistochemistry was positive, and positive cells were distributed in pulp tissue. The deciduous tooth pulp/Matrigel group presented significantly higher microvascular count and optical density of nerve fibers (p < 0.05). Conclusions: This study indicated that that Matrigel can promote primary teeth pulp viability after transplantation.

Matrigel; Dental pulp transplantation; Pulp regeneration

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